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SureSilencing™ siRNA Negative Control (NEG3)

Catalog Numbers
SIH665861ASureSilencing™ siRNA Negative Control (NEG3) for Human, 12.5 nmol/tube
Description
SureSilencing™ siRNA negative control is well designed that has no known gene targets in human, mouse and rat genome. It serves the purpose of distinguishing the sequence-specific silencing from non-specific effects, thus confirming silencing specificity of individual target-specific siRNA. It is also HPLC-purified as the target specific siRNAs. Research Use Only.
Useful Links
Pricing and Ordering User Manual White Paper
Positive Control siRNA siRNA Transfection Reagent
Protocol
Resuspension Instruction Resuspend 12.5 nmol lyophilized siRNA duplex in 625 µl RNase-free ddH2O to get a 20 µM stock solution. Aliquots are recommended when repeated use is needed.

Transfection Instruction
First time users, please refer to the complete protocol in the SureSilencing™ siRNA User Manual.

  • For 96-well transfection, on the day of transfection, dilute SureFECT 0.1 - 0.3 µl to 37.7 - 37.9 µl Opti-MEM I medium for each well.
  • Mix 2-6 pmol (2µl) of siRNA and the negative control siRNA into 38ul of Opti-MEM™ I diluted SureFECT. Mix gently.
  • Incubate siRNA-SureFECT™ mix for 20 min at room temperature.
  • Add 160 ul cells suspension (prepared during the incubation) at 8000 - 12000 cells/160µl in regular culture media to each well of 96-well plate. Mix gently.
  • Incubate the cells at 37 ºC in a CO2 incubator for 24 to 72 hours.
  • Determine the target gene knock down usually at 48 hours after transfection with real-time PCR.
  • Determine the phenotype change at the time point you optimized with proper reagent and/or equipments.
Materials Included

Please check the kit components immediately after you receive this package. SABiosciences is only responsible for missing items reported within two (2) business days of receipt.

Kit Contents:
Component Specification Quantity
siRNA SureSilencing siRNA Negative Control (NEG3), 12.5nmol in each tube  One (1)
InfoSheet SureSilencing siRNA InfoSheet with siRNA sequences  One (1)

Storage Conditions: Store siRNA duplex (either lyophilized or resuspended) at -20º C or below in non-frost-free freezer, avoid contamination of RNases and repeated freeze-thaw cycles. Stable for at least 6 months from the date of shipment.