qBiomarker Somatic Mutation PCR Arrays

The qBiomarker Somatic Mutation PCR Arrays are designed to analyze a panel of somatic mutations reported in the important genes related to a biological pathway. The mutations are selected from comprehensive curated databases (such as the COSMIC database) and literature reviews based on their clinical or functional relevance and frequency of occurrence.

qBiomarker Somatic Mutation PCR Arrays:

Why qBiomarker Somatic Mutation PCR Arrays?

• Pathway Focused: Profile the somatic mutation status of important genes relevant to a major signal transduction pathways.
• Simple and Accurate: Simple real-time PCR procedure provides high sensitivity and wide dynamic range.
• Designed for Routine Use: Bring somatic mutation profiling to almost any lab with a real-time PCR instrument.

How qBiomarker Somatic Mutation PCR Arrays/Assays Work

The procedure involves DNA extraction (QIAGEN QIAamp DNA Mini Kit or FFPE Tissue Kit is recommended), an optional amplification (QIAGEN REPLI-g kit or REPLI-g UltraFast kit is recommended) step for DNA isolated from fresh samples, qPCR detection on qBiomarker Somatic Mutation PCR Arrays or Assays, and data analysis (using the qBiomarker Somatic Mutation Data Analysis Template). An optional DNA sample QC step immediately before the detection array or assay setup allows the user to qualify the DNA samples.

To complete the Somatic Mutation PCR Array procedure, start with 5 to 10ng genomic DNA isolated from fresh tissues, or as low as 200 ng DNA from FFPE sections. DNA from fresh tissues can be uniformly amplified using QIAGEN REPLI-g UltraFast Kit. Then, mix your DNA with the included ready-to-use qBiomarker Probe PCR Master Mixes and aliquot the mixture into each well of the same plate containing pre-dispensed gene-specific primer and hydrolysis probe sets. By performing real-time PCR, the mutation status of a particular sample is determined by comparing the allele specific Ct values between your test sample and a wild-type control sample (see qBiomarker Somatic Mutation Data Analysis section for detailed principles).

Each array contains a panel of 5' nuclease assays for a stringently selected set of pathway focused somatic mutations, gene copy number controls, and PCR quality controls. The PCR Arrays are available in both 96-well and 384-well plate formats, containing either one or four replicates, respectively, of the 96-assay set panel. SABiosciences' qBiomarker Somatic Mutation Assays and qBiomarker Probe Master Mixes have been pre-optimized hand-in-hand for 5' nuclease based real-time RT-PCR detection. The simplicity of the qBiomarker Somatic Mutation PCR Array format and operating procedure allows routine somatic mutation profiling in any research laboratory with access to real-time PCR instruments.

Example Layout of Pathway-Focused qBiomarker Cancer Somatic Mutation PCR Array (96-well format)

The qBiomarker Somatic Mutation PCR Arrays & Assays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.