qBiomarker Somatic Mutation PCR Arrays
The qBiomarker Somatic Mutation PCR Arrays are designed to analyze a panel of
somatic mutations reported in the important genes related to a biological
pathway. The mutations are selected from comprehensive curated databases (such
as the COSMIC database) and literature reviews based on their clinical or
functional relevance and frequency of occurrence.
qBiomarker Somatic Mutation PCR Arrays:
Why qBiomarker Somatic Mutation PCR Arrays?
- Pathway Focused: Profile the somatic mutation status of important
genes relevant to a major signal transduction pathways.
- Simple and Accurate: Simple real-time PCR procedure provides high
sensitivity and wide dynamic range.
- Designed for Routine Use: Bring somatic mutation profiling to
almost any lab with a real-time PCR instrument.
How qBiomarker Somatic Mutation PCR Arrays/Assays Work
The procedure involves DNA extraction (QIAGEN QIAamp DNA Mini Kit or FFPE
Tissue Kit is recommended), an optional amplification (QIAGEN REPLI-g kit or
REPLI-g UltraFast kit is recommended) step for DNA isolated from fresh samples,
qPCR detection on qBiomarker Somatic Mutation PCR Arrays or Assays, and data
analysis (using the qBiomarker Somatic Mutation Data Analysis Template). An
optional DNA sample QC step immediately before the detection array or assay
setup allows the user to qualify the DNA samples.
To complete the Somatic Mutation PCR Array procedure, start with 5
to 10ng genomic DNA isolated from fresh tissues, or as low as 200 ng DNA from
FFPE sections. DNA from fresh tissues can be uniformly amplified using QIAGEN
REPLI-g UltraFast Kit. Then, mix your DNA with the included ready-to-use
qBiomarker Probe PCR Master Mixes and aliquot the mixture into each well of the
same plate containing pre-dispensed gene-specific primer and hydrolysis probe
sets. By performing real-time PCR, the mutation status of a particular sample is
determined by comparing the allele specific Ct values between your test sample
and a wild-type control sample (see qBiomarker Somatic Mutation Data Analysis
section for detailed principles).
Each array contains a panel of 5' nuclease assays for a stringently selected
set of pathway focused somatic mutations, gene copy number controls, and PCR
quality controls. The PCR Arrays are available in both 96-well and 384-well
plate formats, containing either one or four replicates, respectively, of the
96-assay set panel.
SABiosciences' qBiomarker Somatic Mutation Assays and qBiomarker Probe Master
Mixes have been pre-optimized hand-in-hand for 5' nuclease based real-time
RT-PCR detection. The simplicity of the qBiomarker Somatic
Mutation PCR Array format and operating procedure allows routine somatic
mutation profiling in any research laboratory with access to real-time PCR
Example Layout of Pathway-Focused qBiomarker Cancer Somatic Mutation PCR Array (96-well format)
The qBiomarker Somatic Mutation PCR Arrays & Assays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.