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FAQs : Service Core

Real-time PCR and PCR Array Technical FAQ
Q: What is the turnaround time on a project?
A:
PCR Array Analysis:
The typical turn-around time for a 10 sample, 2 pathway project is 3 days.
SYBR Green-based Q-RT-PCR Analysis:
The typical turn-around time for a 10 sample project is 2 days.
miRNA Expression Analysis:
The typical turn-around time for a 10 sample project is 3 days.
Small Sample PCR Array Analysis:
The typical turnaround time for a 10-sample project is approximately three business days for cataloged arrays and three weeks for non-cataloged arrays.
Illumina Genotyping:
The typical turn-around time for a 500 sample project is less than 30 days.
Illumina Gene Expression:
The typical turn-around time for a 24 sample project is less than 5 days.
DNA Methylation Analysis:
The typical turn-around time for a 10 sample project is 3 days.
FFPE Sample Analysis:
The typical turnaround time for a 10-sample project is approximately three weeks.

Q: How do I receive my data?
A: SABiosciences can deliver the data via email or a secure FTP server.

Q: What will my data look like?
A: We have provided Sample Data Examples on each of the individual service pages. If there is not an example data set, please contact technical service at support@sabiosciences.com and an example data set will be provided.

Q: How much RNA do I need to send?
A: PCR Array and Q-RT-PCR Assays: SABiosciences prefers a minimum of 2 micrograms in a minimum volume of 5 microliters. If the customer supplies concentration and quality data then the minimal amount of RNA is 1 microgram in no less than 2 microliters.

Note: For samples < 1 microgram and > 1 nanogram pre-amplification reactions may be used. The Custom Service Core can use SABiosciences's RT² NanoPreAMP kits for these small samples.

Illumina Gene Expression: SABiosciences prefers a minimum of 1 microgram for each sample. If less than the recommend amount of RNA is available, contact a Technical Support representative by email at support@sabiosciences.com to make special arrangements.


Q: What should my RNA be dissolved in?
A: Dissolved in RNase-free water at concentration greater than 0.5 mg/ml. Report actual concentration as well as A260/A280 and A260/A230 ratios.

Q: What are the requirements for submitting cells, tissue, blood, FFPE samples, etc.?
A:
Cells:
  • Provide at least 0.5 million (106) cells for each sample.
  • Specify the number of cells on the order form.
  • Snap freeze the cell pellets in liquid nitrogen.
  • Store samples frozen at -80 ēC, and ship on dry ice
Tissue:
  • If available or known, provide at least 50 mg of tissue for each sample.
  • If available or known, specify the weight of the tissue on the order form.
  • Snap freeze tissues in liquid nitrogen.
  • Alternatively, store and ship in the RNA stabilization reagent, RNAlater(Ambion, catalog number 7020) according to the manufacturer's instructions.
  • Store samples frozen at -80 ēC, and ship on dry ice.
Blood:
  • Please contact a Technical Support representative.
Others:
  • Please contact a Technical Support representative.
Specialized RNA Isolation:
Formalin-Fixed Paraffin-Embedded (FFPE):
  • Please consult a Technical Support representative before isolating and submitting samples.
  • For slide-mounted tissue sections, submit at least two slides with at least 5- to 10-micron thick sections. More slides may be requested from certain tissue sources, if available.
  • For freshly cut sections of whole FFPE blocks, submit three to four 20-micron thick sections.
  • Specify fixation procedure and age of samples.
  • Store and ship at ambient temperature.
Laser Capture Microdissection (LCM):
  • Please consult a Technical Support representative before isolating and submitting samples.
  • Capture cells on Arcturus CapsureŽ LCM caps (catalog number LCM0211).
  • Store samples frozen at -80 ēC, and ship on dry ice.
Fine Needle Aspiration Biopsies (FNAB) and Other Small Samples:
  • Please consult a Technical Support representative before isolating and submitting samples.
  • Use the instructions listed above for Standard RNA Isolation from your biological sample.
For all samples:
  • Obtain a sample submission form from a Technical Sales representative.
  • Complete the form and include it with the shipment of your samples.
  • Include the amount of material and its storage conditions.
  • Sign and date the form.
  • Follow the same directions for the shipment of RNA [here] for shipping your samples.
Once we isolate RNA from your samples, we use our standard in-house Quality Control assays to check the yield and quality of the RNA. The results of these tests largely depend on how carefully the samples were collected, stored, stabilized, and shipped. SABiosciences makes no claims or guarantees that RNA of sufficient quality and quantity will be isolated form the samples that you provide us.

Q: How should I prepare my sample before shipping?
A:
Shipment: US Customers:
Ship samples overnight on at least 10 pounds (lbs) of dry ice.
Customers located on the Maryland NIH campuses may request us to pick up their samples.
Shipment: International Customers:
Ship samples on a Tuesday or on a Friday to arrive within three days.
Ship samples on at least 25 pounds (lbs) of dry ice
Contact our local distributor in your area for help with setting up the shipment.
In other words, use at least enough dry ice to guarantee samples arrive frozen. If samples are shipped improperly, we will request another shipment of samples.

Q: Where do I send my samples?
A: Please address your shipment to:
SABiosciences
Attn: Service Core
6951 Executive Way
Frederick, MD 21703 USA
Phone: 1.301.682.9200