Detailed Technical Resources about SABiosciences Technologies
PCR Arrays and Real-Time PCR
PCR Array: A Simple and Quantitative
Method for Gene Expression Profiling
This paper evaluates the performance of the latest technique for monitoring the
expression of a panel of pathway- or disease focused genes: the RT² Profiler PCR
Array System. It presents scientific data showing that PCR Arrays have the
sensitivity, reproducibility, and specificity expected from real-time PCR
PCR Array Application Examples:
Toxicology, Oncology, and Immunology Research
This paper provides several application examples for the latest technique for monitoring the expression of a panel of pathway- or disease focused genes: the RT² Profiler PCR Array System. It explores the use of the PCR Arrays in immunology, cancer, and toxicology research.
Biomarker Discovery from Paraffin
In this article, we present the successful principles and
results of the new RT² FFPE PreAMP™ technology. This technology combines a
novel RNA extraction protocol that exploits thermodynamic and structural
characteristics of FFPE RNA with a powerful preamplification step of a
pathway-focused set of genes for real-time gene expression analysis.
A Systematic Guideline for
Developing Real-time PCR Primers
This paper summarizes the important
principles that we have learned after designing real-time PCR primer assays for
over 14,000 genes. Critical steps in designing and validating real-time PCR
primers and the solutions to potential problems are described.
miScript miRNA PCR Arrays
miRNA biomarker discovery - overcoming limiting sample material
microRNAs (miRNAs) exhibit a tightly regulated spatial and temporal pattern of expression
during development and differentiation. Furthermore, miRNAs have been shown to be aberrantly
expressed in cancer and other diseases, and may prove to be excellent diagnostic, theranostic, and
prognostic biomarkers. Often the most valuable and informative samples — such as laser-captured
samples, circulating tumor cells, or extracellular miRNA in body fluids — are the hardest to obtain
in amounts sufficient for detailed miRNome profiling. We present an integrated, PCR-based
system that reduces the amount of sample required for full miRNome profiling by several orders
of magnitude and provides unparalleled reproducibility and precision. This advance enables
detailed miRNA analysis on the smallest of samples and opens up new possibilities for biomarker
profiling from blood using the miScript miRNA PCR Arrays - challenges and
The discovery of stable miRNA species circulating in blood has led to increased
research focus on disease-related variations in serum and plasma miRNA
expression and the possibility that such variations could serve as noninvasive
biomarkers for disease. Working with serum and plasma miRNA presents various
challenges in purification and characterization. In this paper, we outline
QIAGEN recommendations for robust purification and quantification, as well as
reliable data normalization and analysis.
Did Your RNAi Experiment Work?! Reliably Validating RNA Interference with Real-Time PCR
This white paper addresses the sources of variation the must be dealt with in validating RNAi. It also describes how the design algorithm for the SureSilencing shRNA Plasmids has twice the success rate of a more commonly used resource, and how it can guarantee the delivery of two successful designs per gene.
Cignal Reporter Assay Kit: A High
Performance Tool for Assessing the Functions of Genes, Biologics, and Small
This paper describes the development of the Cignal Reporter Assays, and
evaluates their performance. Application examples highlighting the utility of
these assays for understanding gene function, as well as determining mechanisms
of action of proteins, peptides, ligands, and small molecule compounds are
Somatic Mutation Arrays
Rapid and accurate cancer somatic mutation profiling with the qBiomarker Somatic Mutation PCR Array
Somatic mutations are associated with cancer, and QIAGEN has now developed real-time PCR-based qBiomarker Somatic Mutation PCR Arrays for pathway- and disease-focused somatic mutation profiling. This white paper describes the technological principles of the qBiomarker Somatic Mutation PCR Arrays as well as the tests performed to assess their performance.
DNA Methylation Arrays
EpiTect Methyl II PCR Array System: A simple tool for screening regional DNA methylation of a large number of genes or samples without bisulfite conversion
This paper discusses the basic principles of the EpiTect Methyl II PCR System, a novel technology enabling simple, fast, and reliable screening of CpG island DNA methylation, without Bisulfite conversion of DNA, for a large number of genes or samples simultaneously, using MethylScreenTM technology. It also shows performance, verification, and application data to demonstrate the System’s robust potential for methylation profiling in various biological systems and for screening of DNA methylation biomarkers.